General Cell Based Assays
Meso Scale Discovery RPLEX MSE Osteoprotegerin ASY
The Meso Scale Discovery R-PLEX Mouse Osteoprotegerin Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of mouse osteoprotegerin (OPG) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The assay has a lower limit of detection of 0 04 ng/mL for mouse OPG and a typical assay time of 3 5 hours This assay enables researchers to accurately quantify circulating OPG levels in mouse models
Eurofins Discoverx PathHunter Panitumumab Bioassay Kit, 2-Plate. (93-1051Y3-00093)
The PathHunter Panitumumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses EGF-induced dimerization of ErbB2 and EGFR receptors. This product uses EFC technology. The cell line included overexpresses ErbB2 target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of ErbB2 and EGFR, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter TrkC Bioassay Kit, 2-Plate. (93-0464Y3-00077)
The PathHunter TrkC Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. NT-3) based activation of TrkC receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged TrkC target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress mCXCR3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0553E2MCP0L)
The PathHunter mCXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of mCXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR3 and EA-tagged β-Arrestin-2. Activation of mCXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1A CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0696E2CP0L)
The PathHunter HTR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1A and EA-tagged β-Arrestin-2. Activation of HTR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mPTAFR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0614E2MCP2L)
The PathHunter mPTAFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PAF) based activation of mPTAFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTAFR and EA-tagged β-Arrestin-2. Activation of mPTAFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress c-KIT Functional Assay, 2-Plate Kit. (93-0853E3CP16M)
The PathHunter eXpress c-KIT Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-KIT dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged c-KIT and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the cynomolgus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress C5AR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0777E3CP5M)
The PathHunter C5AR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of C5AR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged C5AR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting C5AR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPBWR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0638E2CP2M)
The PathHunter NPBWR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptides B/W) based activation of NPBWR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPBWR2 and EA-tagged β-Arrestin-2. Activation of NPBWR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CNR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0183E2CP2L)
The cAMP Hunter CNR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of CNR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CNR2, a naturally Gi/Go coupled GPCR. Upon activation of CNR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL6RA/6ST Bioassay Kit, 10-Plate. (93-1045Y3-00044)
The PathHunter IL6RA/IL6ST Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-6-induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6R target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Innovative Research Inc Hmn uPA Rcmb LMW 100ug
Human Urokinase (uPA) Recombinant Low Molecular Weight 100ug - Human Urokinase (uPA) Recombinant Low Molecular Weight from Innovative Research has been recombinantly produced in insect cells and purified using a combination of conventional and affinity chromatography. This is a frozen liquid buffered in 0.05M Sodium Acetate; 0.1M NaCl; 1mM EDTA; pH 5.0 and a purity of 95%. It is useful as a positive control for ELISA and western blot and and other applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis. Purified from auto-catalytic digestion of recombinant human urokinase produced in insect cells. Active two-chain low molecular weight human urokinase contains the proteinase domain and is separated from the amino terminal fragment by cleavage at the Lys135-Lys136 bond.
Abcam N epsilon (Carboxyethyl) Lysine (CEL) Assay Kit
N epsilon (Carboxyethyl) Lysine (CEL) Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter Human CXCL11 (CXCR3) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human CXCL11 (CXCR3) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CXCR3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress GIPR CHO-K1 GPCR Assay, 2-Plate Kit. (95-0146E2CP2M)
The cAMP Hunter GIPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GIPR, a naturally Gs coupled GPCR. Upon activation of GIPR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.