General Cell Based Assays
Eurofins Discoverx PathHunter Human ADRA2B β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human ADRA2B β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure ADRA2B (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Montana Molecular Human C-C Chemokine Receptor Type 5 (CCR5) BacMam, 30mL
Use this assay to express the human C-C Chemokine receptor 5 (CCR5) in your cells of interest. BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. BacMam expression is easily titrateable to suit your experimental needs.
Eurofins Discoverx PathHunter eXpress GPR50 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0648E2CP1L)
The PathHunter GPR50 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR50 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR50 and EA-tagged β-Arrestin-2. Activation of GPR50 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR2 Total GPCR Internalization Assay, 10pl Kit. (93-0730E3CP0L)
The PathHunter HCRTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress AGTRL1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0147E2CP2M)
The cAMP Hunter AGTRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type AGTRL1, a naturally Gi/Go coupled GPCR. Upon activation of AGTRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter TrkB-P75 Bioassay Kit, 10-Plate. (93-0530Y3-00076)
The PathHunter TrkB-P75 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. BDNF) induced dimerization of TrkB-P75 receptors. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Upon ligand-induced dimerization of TrkB-P75, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress mUTR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0525E2MCP0L)
The PathHunter mUTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. urotensin II) based activation of mUTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mUTR2 and EA-tagged β-Arrestin-2. Activation of mUTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mGLP1R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0478E2MCP0L)
The PathHunter mGLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGLP1R and EA-tagged β-Arrestin-2. Activation of mGLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human VEGF-a ASY
The Meso Scale Discovery U-PLEX Human VEGF-A Assay Kit is a multiplex immunoassay for measuring VEGF-A in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and broad dynamic range with low sample volume The assay offers high specificity and low cross-reactivity It includes a plate with capture antibodies detection antibodies with electrochemiluminescent labels calibrator diluent wash buffer read buffer and a 10-point VEGF-A standard curve The kit is customizable with a 3 5-hour testing time suitable for biomarker research drug discovery and clinical research
Eurofins Discoverx PathHunter Human IL-13 (IL4R/IL13RA1) Dimerization Cell-Based Assay Kit (HEK 293)
The PathHunter Human IL-13 (IL4R/IL13RA1) Stable Cell Line Dimerization Assay (HEK 293) measures ligand-induced dimerization of IL4R/IL13RA1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress CCR10 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0276E3CP5M)
The PathHunter CCR10 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g.CCL27/CTACK ) based activation of CCR10 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR10 and EA-tagged β-Arrestin-2. Activation of CCR10 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Insulin Lispro Bioassay Kit, 2pl. (93-0466Y3-00009)
The PathHunter Insulin Lispro Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses insulin or insulin lispro based activation of INSRb RTK receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery U-PLEX Rat Leptin ASY
The Meso Scale Discovery U-PLEX Rat Leptin Assay Kit is a multiplex immunoassay that measures leptin levels in rat serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with ruthenium calibrators and controls The assay requires a small sample volume and provides results in as little as 3 5 hours With minimal hands-on time this kit allows for the rapid accurate quantification of rat leptin
BioVendor, LLC Sircol; Dye & Dye Release Reagents (2 x 110 ml, 2 x 150 ml). CONTAINS: Sircol; Dye Reagent (2 x 110 ml) AND Sircol™ Dye Release Reagents (2 x 150 ml). Biocolor Item # S1500
Sircol; Dye & Dye Release Reagents (2 x 110 ml, 2 x 150 ml). CONTAINS: Sircol; Dye Reagent (2 x 110 ml) AND Sircol™ Dye Release Reagents (2 x 150 ml). Sircol™ Collagen Assay components. Biocolor Item # S1500
Eurofins Discoverx PathHunter eXpress CMKLR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0313E2CP0L)
The PathHunter CMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CMKLR1 and EA-tagged β-Arrestin-2. Activation of CMKLR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.